An ignored variable: solution preparation temperature in protein crystallization.
Identifieur interne : 000153 ( Main/Exploration ); précédent : 000152; suivant : 000154An ignored variable: solution preparation temperature in protein crystallization.
Auteurs : Rui-Qing Chen [République populaire de Chine] ; Qin-Qin Lu [République populaire de Chine] ; Qing-Di Cheng [République populaire de Chine] ; Liang-Bo Ao [République populaire de Chine] ; Chen-Yan Zhang [République populaire de Chine] ; Hai Hou [République populaire de Chine] ; Yong-Ming Liu [République populaire de Chine] ; Da-Wei Li [République populaire de Chine] ; Da-Chuan Yin [République populaire de Chine]Source :
- Scientific reports [ 2045-2322 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Endopeptidase K, Lysozyme, Protéines, Protéines végétales, Solutions.
- Cristallisation, Température.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Endopeptidase K, Muramidase, Plant Proteins, Proteins, Solutions.
- Crystallization, Temperature.
Abstract
Protein crystallization is affected by many parameters, among which certain parameters have not been well controlled. The temperature at which the protein and precipitant solutions are mixed (i.e., the ambient temperature during mixing) is such a parameter that is typically not well controlled and is often ignored. In this paper, we show that this temperature can influence protein crystallization. The experimental results showed that both higher and lower mixing temperatures can enhance the success of crystallization, which follows a parabolic curve with an increasing ambient temperature. This work illustrates that the crystallization solution preparation temperature is also an important parameter for protein crystallization. Uncontrolled or poorly controlled room temperature may yield poor reproducibility in protein crystallization.
DOI: 10.1038/srep07797
PubMed: 25597864
PubMed Central: PMC4297974
Affiliations:
Links toward previous steps (curation, corpus...)
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Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Qing Di" sort="Cheng, Qing Di" uniqKey="Cheng Q" first="Qing-Di" last="Cheng">Qing-Di Cheng</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author><author><name sortKey="Ao, Liang Bo" sort="Ao, Liang Bo" uniqKey="Ao L" first="Liang-Bo" last="Ao">Liang-Bo Ao</name><affiliation wicri:level="1"><nlm:affiliation>Advanced Materials Test Center, School of Mechanics, Civil Engineering and Architecture, Northwestern Polytechnical University, Xi'an 710129, Shaanxi, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Advanced Materials Test Center, School of Mechanics, Civil Engineering and Architecture, Northwestern Polytechnical University, Xi'an 710129, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Chen Yan" sort="Zhang, Chen Yan" uniqKey="Zhang C" first="Chen-Yan" last="Zhang">Chen-Yan Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author><author><name sortKey="Hou, Hai" sort="Hou, Hai" uniqKey="Hou H" first="Hai" last="Hou">Hai Hou</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Yong Ming" sort="Liu, Yong Ming" uniqKey="Liu Y" first="Yong-Ming" last="Liu">Yong-Ming Liu</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author><author><name sortKey="Li, Da Wei" sort="Li, Da Wei" uniqKey="Li D" first="Da-Wei" last="Li">Da-Wei Li</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author><author><name sortKey="Yin, Da Chuan" sort="Yin, Da Chuan" uniqKey="Yin D" first="Da-Chuan" last="Yin">Da-Chuan Yin</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Crystallization (MeSH)</term><term>Endopeptidase K (chemistry)</term><term>Muramidase (chemistry)</term><term>Plant Proteins (chemistry)</term><term>Proteins (chemistry)</term><term>Solutions (chemistry)</term><term>Temperature (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cristallisation (MeSH)</term><term>Endopeptidase K (composition chimique)</term><term>Lysozyme (composition chimique)</term><term>Protéines (composition chimique)</term><term>Protéines végétales (composition chimique)</term><term>Solutions (composition chimique)</term><term>Température (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Endopeptidase K</term><term>Muramidase</term><term>Plant Proteins</term><term>Proteins</term><term>Solutions</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Endopeptidase K</term><term>Lysozyme</term><term>Protéines</term><term>Protéines végétales</term><term>Solutions</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Crystallization</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cristallisation</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Protein crystallization is affected by many parameters, among which certain parameters have not been well controlled. The temperature at which the protein and precipitant solutions are mixed (i.e., the ambient temperature during mixing) is such a parameter that is typically not well controlled and is often ignored. In this paper, we show that this temperature can influence protein crystallization. The experimental results showed that both higher and lower mixing temperatures can enhance the success of crystallization, which follows a parabolic curve with an increasing ambient temperature. This work illustrates that the crystallization solution preparation temperature is also an important parameter for protein crystallization. Uncontrolled or poorly controlled room temperature may yield poor reproducibility in protein crystallization. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25597864</PMID><DateCompleted><Year>2016</Year><Month>02</Month><Day>09</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>5</Volume><PubDate><Year>2015</Year><Month>Jan</Month><Day>19</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>An ignored variable: solution preparation temperature in protein crystallization.</ArticleTitle><Pagination><MedlinePgn>7797</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/srep07797</ELocationID><Abstract><AbstractText>Protein crystallization is affected by many parameters, among which certain parameters have not been well controlled. The temperature at which the protein and precipitant solutions are mixed (i.e., the ambient temperature during mixing) is such a parameter that is typically not well controlled and is often ignored. In this paper, we show that this temperature can influence protein crystallization. The experimental results showed that both higher and lower mixing temperatures can enhance the success of crystallization, which follows a parabolic curve with an increasing ambient temperature. This work illustrates that the crystallization solution preparation temperature is also an important parameter for protein crystallization. Uncontrolled or poorly controlled room temperature may yield poor reproducibility in protein crystallization. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Rui-Qing</ForeName><Initials>RQ</Initials><AffiliationInfo><Affiliation>1] Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China [2] Advanced Materials Test Center, School of Mechanics, Civil Engineering and Architecture, Northwestern Polytechnical University, Xi'an 710129, Shaanxi, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Qin-Qin</ForeName><Initials>QQ</Initials><AffiliationInfo><Affiliation>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Qing-Di</ForeName><Initials>QD</Initials><AffiliationInfo><Affiliation>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ao</LastName><ForeName>Liang-Bo</ForeName><Initials>LB</Initials><AffiliationInfo><Affiliation>Advanced Materials Test Center, School of Mechanics, Civil Engineering and Architecture, Northwestern Polytechnical University, Xi'an 710129, Shaanxi, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Chen-Yan</ForeName><Initials>CY</Initials><AffiliationInfo><Affiliation>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hou</LastName><ForeName>Hai</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Yong-Ming</ForeName><Initials>YM</Initials><AffiliationInfo><Affiliation>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Da-Wei</ForeName><Initials>DW</Initials><AffiliationInfo><Affiliation>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yin</LastName><ForeName>Da-Chuan</ForeName><Initials>DC</Initials><AffiliationInfo><Affiliation>Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>01</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011506">Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012996">Solutions</NameOfSubstance></Chemical><Chemical><RegistryNumber>53850-34-3</RegistryNumber><NameOfSubstance UI="C003427">thaumatin protein, plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.17</RegistryNumber><NameOfSubstance UI="D009113">Muramidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.21.64</RegistryNumber><NameOfSubstance UI="D019286">Endopeptidase K</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003460" MajorTopicYN="Y">Crystallization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019286" MajorTopicYN="N">Endopeptidase K</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009113" MajorTopicYN="N">Muramidase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011506" MajorTopicYN="N">Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012996" MajorTopicYN="N">Solutions</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>09</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>12</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>1</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>1</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>2</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25597864</ArticleId><ArticleId 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sortKey="Hou, Hai" sort="Hou, Hai" uniqKey="Hou H" first="Hai" last="Hou">Hai Hou</name><name sortKey="Li, Da Wei" sort="Li, Da Wei" uniqKey="Li D" first="Da-Wei" last="Li">Da-Wei Li</name><name sortKey="Liu, Yong Ming" sort="Liu, Yong Ming" uniqKey="Liu Y" first="Yong-Ming" last="Liu">Yong-Ming Liu</name><name sortKey="Lu, Qin Qin" sort="Lu, Qin Qin" uniqKey="Lu Q" first="Qin-Qin" last="Lu">Qin-Qin Lu</name><name sortKey="Yin, Da Chuan" sort="Yin, Da Chuan" uniqKey="Yin D" first="Da-Chuan" last="Yin">Da-Chuan Yin</name><name sortKey="Zhang, Chen Yan" sort="Zhang, Chen Yan" uniqKey="Zhang C" first="Chen-Yan" last="Zhang">Chen-Yan Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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